External and internal triggers of cell death in yeast

In recent years, yeast was confirmed as a useful eukaryotic model system to decipher the complex mechanisms and networks occurring in higher eukaryotes, particularly in mammalian cells, in physiological as well in pathological conditions. This article focuses attention on the contribution of yeast in the study of a very complex scenario, because of the number and interconnection of pathways, represented by cell death. Yeast, although it is a unicellular organism, possesses the basal machinery of different kinds of cell death occurring in higher eukaryotes, i.e., apoptosis, regulated necrosis and autophagy. Here we report the current knowledge concerning the yeast orthologs of main mammalian cell death regulators and executors, the role of organelles and compartments, and the cellular phenotypes observed in the different forms of cell death in response to external and internal triggers. Thanks to the ease of genetic manipulation of this microorganism, yeast strains expressing human genes that promote or counteract cell death, onset of tumors and neurodegenerative diseases have been constructed. The effects on yeast cells of some of these genes are also presented

Hypothesis: is yeast a clock model to study the onset of humans aging phenotypes?

In this paper we report the growth and aging of yeast colonies derived from single cells isolated by micromanipulation and seeded one by one on separated plates to avoid growth interference by surrounding colonies. We named this procedure clonal life span, and it could represent a third way of studying aging together with the replicative life span and chronological life span. In this study we observed over time the formation of cell mass similar to the human "senile warts" (seborrheic keratoses), the skin lesions that often appear after 30 years of life and increase in number and size over the years. We observed that similar signs of aging appear in yeast colonies after about 27 days of growth and increase during aging. In this respect we hypothesize to use yeast as a clock to study the onset of human aging phenotypes

Non-relativistic limit of scalar and Dirac fields in curved spacetime

We give from first principles the non-relativistic limit of scalar and Dirac fields in curved spacetime. We aim to find general relativistic corrections to the quantum theory of particles affected by Newtonian gravity, a regime nowadays experimentally accessible. We believe that the ever-improving measurement accuracy and the theoretical interest in finding general relativistic effects in quantum systems require the introduction of corrections to the Schr\"{o}dinger-Newtonian theory. We rigorously determine these corrections by the non-relativistic limit of fully relativistic quantum theories in curved spacetime. For curved static spacetimes, we show how a non-inertial observer (equivalently, an observer in the presence of a gravitational field) can distinguish a scalar field from a Dirac field by particle-gravity interaction. We study the Rindler spacetime and discuss the difference between the resulting non-relativistic Hamiltonians. We find that for sufficiently large acceleration, the gravity-spin coupling dominates over the corrections for scalar fields, promoting Dirac particles as the best candidates for observing non-Newtonian gravity in quantum particle phenomenology

Minkowski vacuum in Rindler spacetime and Unruh thermal state for Dirac fields

We consider a free Dirac field in flat spacetime and we derive the representation of the Minkowski vacuum as an element of the Rindler-Fock space. We also compute the statistical operator obtained by tracing away the left wedge. We detail the resulting thermal state for fermionic particles

Frame-dependence of the non-relativistic limit of quantum fields

We study the non-relativistic limit of quantum fields for an inertial and a non-inertial observer. We show that non-relativistic particle states appear as a superposition of relativistic and non-relativistic particles in different frames. Hence, the non-relativistic limit is frame-dependent. We detail this result when the non-inertial observer has uniform constant acceleration. Only for low accelerations, the accelerated observer agrees with the inertial frame about the non-relativistic nature of particles locally. In such a quasi-inertial regime, both observers agree about the number of particles describing quantum field states. The same does not occur when the acceleration is arbitrarily large (e.g., the Unruh effect). We furthermore prove that wave functions of particles in the inertial and the quasi-inertial frame are identical up to the coordinate transformation relating the two frames

Clinical and in vitro efficacy of colistin plus vancomycin and rifampin against colistin-resistant Acinetobacter baumannii causing ventilator-associated pneumonia

We present the case of a patient with ventilator-associated pneumonia (VAP) caused by a pan-resistant Acinetobacter baumannii successfully treated with the combination colistin plus vancomycin plus rifampin, whose in vitro activity was investigated by checkerboard method and killing testing. Furthermore, the serum bactericidal activity (SBA) was assessed. Our case shows that an innovative regimen consisting of colistin plus antimicrobials active only against Gram-positive microorganisms might represent a valid therapeutic option for severe infections caused by colistin-resistant A. baumannii

A systematic study of harnessing low-temperature geothermal energy from oil and gas reservoirs

Mature hydrocarbon fields co-produce significant volumes of water. As the produced water increases over the life of the field, the project's operating costs increase (due to greater water management expenditure), while the oil revenues decrease. Typically, these waste streams of water have temperatures of 65–150 °C. The combination of moderate temperatures and large water volumes may be suitable for electricity generation and/or district heating. Being able to capture the geothermal energy from existing hydrocarbon fields could extend their lifespan by delaying their economic cut-off point. In this paper, mature oil and gas reservoirs worldwide are critically reviewed, where waste heat recovery has already been tested, or its potential identified. A roadmap of screening criteria based on geological, reservoir, production and economic parameters is then proposed, to assess how, where and when low-temperature waste heat recovery is feasible. The roadmap is tested against the Villafortuna–Trecate oil field in Italy, where the aquifer not only provides pressure support to the reservoir, but also represents a natural, in-situ hydrothermal resource. The results suggest that a single-well could recover approximately 25 GWh of electric power over a 10-year period, with an installed capacity of 500 kW

Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumine in Kluyveromyces lactis

KlADH4 is a gene of Kluyveromyces lactis encoding a mitochondrial alcohol dehydrogenase activity which is specifically induced by ethanol. The promoter of this gene was used for the expression of heterologous proteins in K. lactis, a very promising organism which can be used as an alternative host to Saccharomyces cerevisiae due to its good secretory properties. In this paper we report the ethanol-driven expression in K. lactis of the bacterial beta-glucuronidase and of the human serum albumin (HSA) genes under the control of the KlADH4 promoter. In particular, we studied the extracellular production of recombinant HSA (rHSA) with integrative and replicative vectors and obtained a significant increase in the amount of the protein with multicopy vectors, showing that no limitation of KlADH4 trans-acting factors occurred in the cells. By deletion analysis of the promoter, we identified an element (UASE) which is sufficient for the induction of KlADH4 by ethanol and, when inserted in the respective promoters, allows ethanol-dependent activation of other yeast genes, such as PGK and LAC4. We also analyzed the effect of medium composition on cell growth and protein secretion. A clear improvement in the production of the recombinant protein was achieved by shifting from batch cultures (0.3 g/liter) to fed-batch cultures (1 g/liter) with ethanol as the preferred carbon source

Weathering of evaporites: natural versus anthropogenic signature on the composition of river waters

Weathering of evaporites strongly influences the chemistry of continental runoff, making surface waters poorly exploitable for civil uses. In south-central Sicily, this phenomenon is worsened by the occurrence of abandoned landfills of old sulphur and salt mines. The industrial evolution of the Bosco-S. Cataldo mining site leaved two landfills from the early exploitation of a sulphur mine followed by that of a kainite deposit. In particular, the weathering of these landfills leads the dissolved salt (TDS) values up to about 200 g l−1 in the Stincone–Salito Stream waters. This process induces the V, Cr and Fe desorption from sediments and particulates in the aqueous phase under reducing conditions. At the same time, the weathering of salt minerals releases Rb and Cs, originally contained in halite. The overall processes lead to the V, Cr, Fe, Rb and Cs enrichment of waters from the Stincone–Salito Stream system accompanied by a sharp growth of As content, up to about 13 µg l−1, caused by As release from Fe-bearing solids due to the high salinity. Therefore, the scenario of the weathering of Bosco-S. Cataldo mine landfills depicts an environment strongly influenced by effects of the growing salinity and euxinic water conditions where the attained TDS, Eh and pH conditions reduce the natural scavenging capability of the interested river system, favouring a growth of residence time of toxic elements in river waters

Clinical and in vitro efficacy of colistin plus vancomycin and rifampin against colistin-resistant Acinetobacter baumannii causing ventilator-associated pneumonia

We present the case of a patient with ventilator-associated pneumonia (VAP) caused by a pan-resistant Acinetobacter baumannii successfully treated with the combination colistin plus vancomycin plus rifampin, whose in vitro activity was investigated by checkerboard method and killing testing. Furthermore, the serum bactericidal activity (SBA) was assessed. Our case shows that an innovative regimen consisting of colistin plus antimicrobials active only against Gram-positive microorganisms might represent a valid therapeutic option for severe infections caused by colistin-resistant A. baumannii